Insertion junction: CLIP2.026952_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.026952
Chromosome:chromosome 16
Location:1030117
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g649150 (1 of 1) PF13414//PF13920 - TPR repeat (TPR_11) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCCAATGATCGCAACCCAGGGCAGCGGCTTTCCGCTACAGGCCCAGG
Internal bar code:GTCCGTGGCAACAGTGCGTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1244
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCTCCCTGGACAACATC
Suggested primer 2:AGAGCAACAACCTCCGTCTG