Insertion junction: CLIP2.026980_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026980
Chromosome:chromosome 7
Location:1590620
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325101 (1 of 2) PTHR30336 - INNER MEMBRANE PROTEIN, PROBABLE PERMEASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTGGTGGAAGGGGCTGGTCACCACCGCCACACTCCGCCACCTGCATGG
Internal bar code:ACACAGAGGCTGATAACTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3337
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCGCCACTGCATATTGTC
Suggested primer 2:AGTTGGAAATGTGCGTTGCC