Insertion junction: CLIP2.026989_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.026989
Chromosome:chromosome 4
Location:1278766
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g800460 (1 of 37) IPR009000 - Translation protein, beta-barrel domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAACTCCCCACCACCGCACCGCGGCATCTGCGAGCCCCAACCAGCCAAC
Internal bar code:CGGTTCGGCGCTGAGACCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1150
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGTCCATGCGGAACTTGG
Suggested primer 2:CCAGTCACCCTACAACGCTT