Insertion junction: CLIP2.027005_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.027005
Chromosome:chromosome 13
Location:2186943
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578051 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGCGTGCCACGGGGGGGTACGTCACGGATGCGGGGGCGCCGTATGGT
Internal bar code:GTGTCGTTTTGCGTTCTGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1561
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGGCACCAAGTCCAAACG
Suggested primer 2:TGTCTTCTCAACTCGGGTGC