Insertion junction: CLIP2.027012_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027012
Chromosome:chromosome 12
Location:8608358
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548450 GT90-24,GT90F24 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 24 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAACTTTACTCGTTGGGGTCGGGCACATAACCCCACCCACACCCGTCCG
Internal bar code:CAATATAGTTAGATCTATCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:724
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGTTTGTGGACCAGTGCA
Suggested primer 2:TGTAGCAGCCTTCTTGAGCC