Insertion junction: CLIP2.027029_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027029
Chromosome:chromosome 5
Location:453777
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g243900 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGCTTCTTCTGTCCCTTGTGCTCTCTGACACACCCAATGCATGTG
Internal bar code:GGGGTCCTCGTCTGCGTTCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:372
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAGTCGATCACCTCCAC
Suggested primer 2:CCAAACCCCTCCTTCTCCAC