Insertion junction: CLIP2.027032_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027032
Chromosome:chromosome 6
Location:3280114
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g277250 (1 of 32) IPR006502 - Protein of unknown function PDDEXK-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCGGGAGCTGCGCTTTCGCCGCGGCGGCGATGGCAGTAGCAGCGGCAG
Internal bar code:TTAGTGGGGAATGGGGACACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3783
LEAP-Seq percent confirming:74.4186
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CATTAATCTGACGCCGCAGC
Suggested primer 2:CGTGGTACAATCCAAGCCCT