Insertion junction: CLIP2.027053_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.027053
Chromosome:chromosome 2
Location:547016
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g076800 ERG4 Delta14-sterol reductase, mitochondrial; (1 of 1) 1.3.1.70 - Delta(14)-sterol reductase / Sterol C14-reductase CDS
Cre02.g076850 (1 of 1) PTHR19854:SF1 - GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1 outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTTCCTCCTTTCCCCATCTCTGTTTAAAGAAAGCTGGATTCAGCGCGA
Internal bar code:TAACAATTGAGATACCAATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2649
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGCTGCCATTACCTACTA
Suggested primer 2:CGAATCCAAAGTACAGCGCG