| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027065 |
| Chromosome: | chromosome 6 |
| Location: | 8151326 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305950 | CSB32,TNP18 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGACCTGCTACAACTGCGGGTGTCAGGAGATGGCCATCAAGCGGCTT |
| Internal bar code: | AGCAGTAGCGGCTATCACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2552 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTATCAACCCAGCCACCT |
| Suggested primer 2: | GGGCCGATTCCATATGAGCA |