| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.027078 |
| Chromosome: | chromosome 16 |
| Location: | 1628610 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654250 | THB6 | Truncated hemoglobin; (1 of 2) PTHR10681:SF17 - PEROXIREDOXIN-6 | CDS |
| Cre16.g654300 | NDK5,RSP23 | (1 of 1) PF00334//PF00612//PF05186 - Nucleoside diphosphate kinase (NDK) // IQ calmodulin-binding motif (IQ) // Dpy-30 motif (Dpy-30); Radial Spoke Protein 23 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTACACGACCTGCGAGGCAAGCGGCCATGGAACCGCGAGGGAGCGCA |
| Internal bar code: | GATGAGAGGAAGTTGGTTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCAAGCTTTCAGGCAAC |
| Suggested primer 2: | CGCGGCGTAATCAAATCGTT |