Insertion junction: CLIP2.027102_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.027102
Chromosome:chromosome 6
Location:1019445
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256900 SND1C SAND domain-encoding short ORF; (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCCCGGCGAGGGTGCCGAGTTGCGCGGCGCCGCAGGCCGTGCCACTTG
Internal bar code:TGTTAGTTAGGTGGAATATTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2121
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACCTCCGTATCGTGAAG
Suggested primer 2:CGCACCTTGGTTGGGAAAAG