| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027117 |
| Chromosome: | chromosome 12 |
| Location: | 2154990 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509500 | (1 of 1) IPR000595//IPR002048//IPR011992//IPR018490//IPR029530 - Cyclic nucleotide-binding domain // EF-hand domain // EF-hand domain pair // Cyclic nucleotide-binding-like // Cell division control protein 31 | 5'UTR_intron | |
| Cre12.g509550 | PDE5 | (1 of 3) 3.1.4.17//3.1.4.53 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase // 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCAGACCGCTCACCCAGCGGTGGTGCACCTCCCACGGCGCGGCCA |
| Internal bar code: | AAGTTACAAGAGAATACTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1754 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCATATTCGCGTCCTAC |
| Suggested primer 2: | GGTGCGGGATCTCGAGTAAG |