Insertion junction: CLIP2.027125_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.027125
Chromosome:chromosome 4
Location:2194801
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g220100 (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTCACCAGAGGGTGAGGCCAGCGACCGGCCTCGCGGGGCCCTGGCGGG
Internal bar code:AAGAGTATTTATCGAGATATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1722
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTCGTCGAAGCTGCTGTT
Suggested primer 2:TCAACAGTCGGTCGTCACAG