| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.027170 |
| Chromosome: | chromosome 13 |
| Location: | 1506376 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572650 | POB13 | Proteome of basal body 13 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCGCCTCTCCACGCGAGACGCTGTCCTGCAGATGTTCTAGGGCCACG |
| Internal bar code: | ATAATGGGATAAACTATTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1203 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGTGCAGTGTCGGAACC |
| Suggested primer 2: | TGCATCGTCAGAGCAACACT |