| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.027172 |
| Chromosome: | chromosome 13 |
| Location: | 2711773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581801 | (1 of 1) PTHR13198//PTHR13198:SF4 - RING FINGER PROTEIN 25 // E3 UBIQUITIN-PROTEIN LIGASE RNF25 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGTGTGCCTGTCGAAAGGGACGCAGGGTTGCGTGTAGGAGATGCGGT |
| Internal bar code: | ATGGGATTCTCGAGTTATTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1586 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACGATCCCCTCCCCCAT |
| Suggested primer 2: | ATTGGCAGCTTGCTGGGTAT |