| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027179 |
| Chromosome: | chromosome 17 |
| Location: | 4608899 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732300 | GAL1 | (1 of 1) 2.7.1.6 - Galactokinase; Galactose kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGCGCTATCGATGATGCTTCTGACAAGGACGTTTCCTCACATCGGT |
| Internal bar code: | GCATCTTTTCCCATCGCAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGTTATGCGAGTCGGAG |
| Suggested primer 2: | CTTGTTGTGACCTGTTGCGG |