| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.027221 |
| Chromosome: | chromosome 3 |
| Location: | 1522810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151850 | (1 of 1) PF06884 - Protein of unknown function (DUF1264) (DUF1264) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCGGTGCCGCTGCCATCGGTGGCATTGCTGCCAGTGCCCTTCGGCGG |
| Internal bar code: | TTATATAACTGGTTTTCATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTGCGCTGACTGGTGAGA |
| Suggested primer 2: | TGTACTCGATGCCGATCAGC |