| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027231 |
| Chromosome: | chromosome 7 |
| Location: | 5478856 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351050 | VPS23 | (1 of 1) K12183 - ESCRT-I complex subunit TSG101 (TSG101, STP22, VPS23); Subunit of the ESCRT-I complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGACCAGTATTATGGGTTCCGGGGTTTGTGCCCAGTAGCCTGAGTAG |
| Internal bar code: | TGCGTCTTACTCTCTTTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3827 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACACACACCTGCGTCTCC |
| Suggested primer 2: | GTAACCAGCAGCAGCAACAG |