Insertion junction: CLIP2.027249_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027249
Chromosome:chromosome 2
Location:2779344
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094100 NRX1 (1 of 3) K17609 - nucleoredoxin [EC:1.8.1.8] (NXN); Nucleoredoxin 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCGCGGCATTCCCAGCCTCGTCATCGTGGACGGTGCAACTGGCGAGC
Internal bar code:CGTGATAGGGCCTGAGCGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3971
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:98
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTTGAACTGCTCGTCCA
Suggested primer 2:CCGAGGGTGTTTGAGCTCTT