| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.027257 |
| Chromosome: | chromosome 1 |
| Location: | 5775659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGCACTGATGTTTCTTGTTCTGCCTTTCCTCGCCGGCTGCCGACTT |
| Internal bar code: | TCAGGTGGTATCTGATAATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3720 |
| LEAP-Seq percent confirming: | 96.3636 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTGTGGCGTGACCGTAA |
| Suggested primer 2: | TTACTATGGGGATGGCACGC |