Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.027260 |
Chromosome: | chromosome 12 |
Location: | 8871889 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g546200 | RBL5 | Rhomboid-like protein; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCAGCGCTGCTTAGTGGCGCACCTGGCCATCGCATCGCAGCCGGACC |
Internal bar code: | GTGGCCCCGGTGGTGACCCAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1348 |
LEAP-Seq percent confirming: | 97.2222 |
LEAP-Seq n confirming: | 35 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCGTGACTCCGGTATAGG |
Suggested primer 2: | CTCGCGGCTCATAATGGTCT |