| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027260 |
| Chromosome: | chromosome 12 |
| Location: | 8871893 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546200 | RBL5 | Rhomboid-like protein; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAACATGTCAGCGTGCGGCTTCCGGTCTATGTCCTCCAGCCAGTATG |
| Internal bar code: | GTGGCCCCGGTGGTGACCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1186 |
| LEAP-Seq percent confirming: | 24.6154 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCGGCTCATAATGGTCT |
| Suggested primer 2: | GTGCGTGACTCCGGTATAGG |