| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027272 |
| Chromosome: | chromosome 3 |
| Location: | 8146178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200750 | DMC1 | Putative DNA methylase; (1 of 1) IPR027417//IPR029063 - P-loop containing nucleoside triphosphate hydrolase // S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCACATGCTGCAGGACGTGCCGGCAGGTGCGGGCGTGAGGGCGCGGGC |
| Internal bar code: | ACAGGGGTACTCCGACGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2412 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTTGTGAAGCCGTTGAC |
| Suggested primer 2: | GCCCTACGTGTACCTGGAAC |