Insertion junction: CLIP2.027282_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027282
Chromosome:chromosome 5
Location:2874560
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g241500 MOT30 Predicted protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGCTGGCGCGCATGAAGCGCGTGGCTGAGGAGCGCCGTGAGATCGCGG
Internal bar code:CCTGTCACGTATGTGCAGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:665
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGCGACCTGCAATACTC
Suggested primer 2:GGCAACCGTAAACACACCAC