| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027303 |
| Chromosome: | chromosome 2 |
| Location: | 3256826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097227 | Found in Chlamydomonas and Volvox but not strongly conserved elsewhere | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGGTGTGTATCAAATATGAAGTGAAGTGCCGACGTCCAGAGGTAC |
| Internal bar code: | GGTGCACAAACAGTTTTAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 38.8889 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGGACTCACCACCTTGC |
| Suggested primer 2: | TGTGTATCAGGGGAGGGGAG |