Insertion junction: CLIP2.027307_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027307
Chromosome:chromosome 6
Location:5149892
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g283250 POB9 Proteome of basal body 9 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTCATACTTACCCGCGATAAGCCTGGAACCCCACGGGCGACCATTCGG
Internal bar code:ATGTGGAATGAGGTATATAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3708
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GACAGGACCGCAAGTGTGTA
Suggested primer 2:CCAGGTACGGTGTGAAACGA