Insertion junction: CLIP2.027318_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027318
Chromosome:chromosome 6
Location:6418934
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293516 DEGO2 (1 of 4) PTHR22939:SF83 - PROTEASE DO-LIKE 2, CHLOROPLASTIC; Inactive Deg protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGACAAACATACACGCACAGTCTTTGTGCTGGATTTTCCTTGTAATCTA
Internal bar code:GGGTACTATTTGCTGGATTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:403
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGATCGTGTTGCTGGTGGAG
Suggested primer 2:CCCTCCTCCTCCCTTCTTCA