Insertion junction: CLIP2.027327_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.027327
Chromosome:chromosome 16
Location:1187057
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g650600 MST1 Mastigoneme-like flagellar protein; (1 of 4) IPR009030//IPR011641 - Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCGCTGGTGGTGCGGCTGCGGTCTGGAGAATCGTCATCACGCGTAAT
Internal bar code:GTCCAAAGTTTGTAAGCTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1512
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTGCCATCGCCTTCATT
Suggested primer 2:GCGGTTGTTCCTGTTGACAC