| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.027330 |
| Chromosome: | chromosome 12 |
| Location: | 4323252 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518851 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCAGGGGGCGGCGGCGGCGGCGGCAATAGAGGCACCGGCGGCGGATG |
| Internal bar code: | GTAGGCCTCCTTGTAAACTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2838 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACTGCCTGTTGACAAG |
| Suggested primer 2: | CTGTGGCTACCGTTTGGCTA |