Insertion junction: CLIP2.027344_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027344
Chromosome:chromosome 12
Location:9829828
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g546877 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTTGGTGCCTGCGGGGCGCAGTCGCTCGGCGCAGGCTGCTCGGCGCTG
Internal bar code:GCCCTTTTAGCGGGCTTCAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2271
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAGCAATGCCAGTGTGA
Suggested primer 2:TATGCTCGACTTCCTGCGTC