Insertion junction: CLIP2.027357_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.027357
Chromosome:chromosome 2
Location:3089842
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g096000 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACACAACACCAGCCCCAACACCACCGCAATGCAAAGTCAATAAGCAG
Internal bar code:ATATGATGTGATGGCAACTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2085
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATAGAGGAGTGTCCGCA
Suggested primer 2:CGGCGCACTACTGTAGAGAG