| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027373 |
| Chromosome: | chromosome 6 |
| Location: | 5936304 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289950 | HEN2,HEL28 | (1 of 2) K12598 - ATP-dependent RNA helicase DOB1 (MTR4, SKIV2L2); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGAGCTCGTAACCCGATGTCGCAGCGCATGACTAAGCTACGATGGTAA |
| Internal bar code: | GGGTCCTGCTTCAAGTGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3270 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTGGAACTTGTGACGC |
| Suggested primer 2: | ACAGGAATGAGGCATGTGGG |