| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027377 |
| Chromosome: | chromosome 12 |
| Location: | 2202294 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509000 | MAPK3 | Mitogen-Activated Protein Kinase 3; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCCCCAGCGACTTGATGATGAGCTTTAGCTGGTCCACGTAATCCT |
| Internal bar code: | TGTATGGGCGCTGGGGTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2770 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATATGCGACTTCGGCCTG |
| Suggested primer 2: | CTCGTCGACTCCTAAGCCAC |