Insertion junction: CLIP2.027382_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.027382
Chromosome:chromosome 6
Location:3774916
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278170 FAP237,FAS7,FAS16,FLA11 Flagellar Associated Protein 237; (1 of 1) PTHR10900:SF77 - PROTEIN F26E4.7, ISOFORM A CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATCCCCGTGGGCCTCAGTGAGTGGCGGTGGCGCGCCGGGGACACCGGG
Internal bar code:AGACGATATCTTGATGATGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:5512
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGATGATGGACTTGCCC
Suggested primer 2:ACGAACAACCCCAACCTTGT