| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.027400 |
| Chromosome: | chromosome 17 |
| Location: | 6495188 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744497 | (1 of 1) PF09783 - Vacuolar import and degradation protein (Vac_ImportDeg) | CDS | |
| lncRNA_TCONS_00240303 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGACGTGCCGTCGGTGCCTTCCTGCAGCTACCTAATGCCGGGCCGGA |
| Internal bar code: | GTTATGACTCGCACGTACGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACGGGAAACACACAGC |
| Suggested primer 2: | TACTTCTTCTACCGCGGGGA |