| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027408 |
| Chromosome: | chromosome 3 |
| Location: | 3492792 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167250 | (1 of 1) PF05673 - Protein of unknown function (DUF815) (DUF815) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGGCGCGGGTAGCGGCCGCAGCCCCGCGCCACCTCCAGGATGTTGC |
| Internal bar code: | TCGGTTTATTTGCTAAGGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3688 |
| LEAP-Seq percent confirming: | 95.3846 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGTGATTGCACCGCTG |
| Suggested primer 2: | CAATTTCCACCGCATCCACC |