Insertion junction: CLIP2.027409_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.027409
Chromosome:chromosome 16
Location:905826
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648200 CYP744C1,CYP35 (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A); Cytochrome P450, CYP3 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACTACCACTCGGTCACTACCCAACACGAACTAACGTGCATCAGTGT
Internal bar code:CCGGCGAGCTAGCTGCTGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1835
LEAP-Seq percent confirming:89.5833
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACTTCTGTCACGACGTG
Suggested primer 2:TCGTCCTTCATGTCCACTGC