| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027426 |
| Chromosome: | chromosome 1 |
| Location: | 3443552 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022350 | HEL3 | DEAH box ATP-dependent RNA helicase; (1 of 1) K14805 - ATP-dependent RNA helicase DDX24/MAK5 [EC:3.6.4.13] (DDX24, MAK5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGATACCAGCCCAAACTTAACCAAACCCAATGCTGGGCTGGGGAGAT |
| Internal bar code: | ACCCTGACGGTCTATGATTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1828 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCGGTCAGGACAACT |
| Suggested primer 2: | GGATGTGTGTGTGTGTGTGC |