| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027445 |
| Chromosome: | chromosome 16 |
| Location: | 7443031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681238 | (1 of 1) K03650 - tRNA modification GTPase [EC:3.6.-.-] (mnmE, trmE, MSS1) | 5'UTR | |
| Cre16.g681354 | (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCGCTGTTGCCTGCTCTCAGCTCAAATCGCTCTACTACCACGTTG |
| Internal bar code: | AGAGCTTTGAGTTTGGTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3619 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCAGCAATCCGTTAAG |
| Suggested primer 2: | TCGGTGTGGTGAGGAATTGG |