| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027446 |
| Chromosome: | chromosome 17 |
| Location: | 5198170 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736950 | (1 of 1) PF11265 - Mediator complex subunit 25 von Willebrand factor type A (Med25_VWA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGCGCGCCGCGGTCAACGGTAAGCATGGCGCGTTGTGGGTTCAGGAG |
| Internal bar code: | CAAATGTAGGGATACCGAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2872 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCCCAGGGTATACAG |
| Suggested primer 2: | AACGACGTGTTGCATCCTCT |