| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027450 |
| Chromosome: | chromosome 7 |
| Location: | 5309807 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349950 | (1 of 1) K03131 - transcription initiation factor TFIID subunit 6 (TAF6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTGGATGTGTGCAAACAAGCTCTCGTGGCGGTGTGGGGCCGGGGTG |
| Internal bar code: | GACTTGTGCGACTCCGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGTAGTGGGCGGGTTA |
| Suggested primer 2: | ACGCACATACACAAACACGC |