Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.027452 |
Chromosome: | chromosome 10 |
Location: | 4921890 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g453807 | CTPB1 | (1 of 2) PTHR32060:SF5 - PEPTIDASE S41 FAMILY PROTEIN; C-terminal peptidase B | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCCCGAACCACACCACGGAGTCCACGCACCAGCAAATGTCCGTTCA |
Internal bar code: | GAAGGTGGAACAGACTGATATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 766 |
LEAP-Seq percent confirming: | 96.2963 |
LEAP-Seq n confirming: | 26 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCATTTGTGTGTGCTGAGC |
Suggested primer 2: | CCGTCTCTTGCCAAACTCCT |