Insertion junction: CLIP2.027452_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027452
Chromosome:chromosome 10
Location:4921890
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g453807 CTPB1 (1 of 2) PTHR32060:SF5 - PEPTIDASE S41 FAMILY PROTEIN; C-terminal peptidase B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGCCCGAACCACACCACGGAGTCCACGCACCAGCAAATGTCCGTTCA
Internal bar code:GAAGGTGGAACAGACTGATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:766
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATTTGTGTGTGCTGAGC
Suggested primer 2:CCGTCTCTTGCCAAACTCCT