| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.027486 |
| Chromosome: | chromosome 7 |
| Location: | 1615354 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325250 | (1 of 2) PF06977 - SdiA-regulated (SdiA-regulated) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGGATTTCCGCGAACTTGAGGAATTTCTCCAGCTTATAGCTGCCTA |
| Internal bar code: | GCATCGTAGCGGGCGCGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1889 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGCGAGCAACCTAGTA |
| Suggested primer 2: | ACGGGAGGAGCAATGTGATG |