| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.027496 |
| Chromosome: | chromosome 6 |
| Location: | 4662553 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278500 | TMG3 | (1 of 2) K15445 - tRNA (guanine9-N1)-methyltransferase [EC:2.1.1.221] (TRMT10A, TRM10, RG9MTD2); tRNA (guanine-N1)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCTGGTGCGTGGCGGTGACACGAACAATCAGAGAAGGGCGGCAAGGG |
| Internal bar code: | GGCGTGATTTGGCTACGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCATGCTGTACGTTCCC |
| Suggested primer 2: | TGAAGTGGCGCAGGTTATGT |