Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.027505 |
Chromosome: | chromosome 17 |
Location: | 219564 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g697334 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATCTCGCCCGTCTCCAGGTTGTCCATCACCTCGCCCATCTTCGCCTC |
Internal bar code: | GTTTGTATCTTGCAGGTCTGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 479 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 16 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGCAGTGAGTGTGTGTGC |
Suggested primer 2: | ACATGGGCATTGTGGACTGT |