| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027510 |
| Chromosome: | chromosome 6 |
| Location: | 2109698 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266100 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACACAGGTCGCGGCAGCCCTCAGGCACCCACTCGTCCAGGTCGTCC |
| Internal bar code: | CCCTACAGGGCTATCGACTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4229 |
| LEAP-Seq percent confirming: | 52.0833 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTTACAGTGCTGCCTC |
| Suggested primer 2: | CTGCAAATGATCCTCGTGCG |