| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.027510 |
| Chromosome: | chromosome 12 |
| Location: | 9525467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540200 | HEL58 | (1 of 5) K12823 - ATP-dependent RNA helicase DDX5/DBP2 (DDX5, DBP2); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTATAAGGAGTCATCGGCTGCATGGGGTCAAGTCGGCGGGCGTCGGTT |
| Internal bar code: | AATTCAGGGGATCAGGTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1286 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCATCCATGTGTGCCC |
| Suggested primer 2: | GTCTTTCCTGCCTCTCCACC |