| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.027523 |
| Chromosome: | chromosome 7 |
| Location: | 3869774 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339050 | NSG11 | Cofilin/tropomyosin-type actin-binding protein; (1 of 1) K05765 - cofilin (CFL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGTTTGGGTTGGGTTGGGGTTGGGGTGGAGTTGCTATGAATGGCA |
| Internal bar code: | TTACGCGCGTAGGTGGATAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGCGCCTTTCCATCTGG |
| Suggested primer 2: | GACGTGACTGGGGCTACAAA |