| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027527 |
| Chromosome: | chromosome 16 |
| Location: | 7770910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687518 | (1 of 1) K03239 - translation initiation factor eIF-2B subunit alpha (EIF2B1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGTCGGGTAGGGGGAGAGGTCAGGGCGGGCATGCGGGCAGGGTTGG |
| Internal bar code: | TTTACCACGTTTAAGTAGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2253 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATGTGAAGCCCGAACT |
| Suggested primer 2: | CGGGCCTTGCAAATCAAACA |