| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.027558 |
| Chromosome: | chromosome 10 |
| Location: | 4491725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451000 | TRR1 | (1 of 1) K15407 - queuine tRNA-ribosyltransferase subunit QTRTD1 [EC:2.4.2.29] (QTRTD1); Queuine tRNA-ribosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGGTCGCCTCACCATGTGCCCCCCGCTGTGTATGCACACGTGCACC |
| Internal bar code: | TAAGCGGGGGCGCGCTGCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCCTATTGCATGCTGC |
| Suggested primer 2: | GAGTCGGCCGTGATCTGTAG |